Publications

65. Biosynthesis of bacterial meroterpenoid reveals a non-canonical class II terpene cyclase
Wang, Z.; Alsup, T. A.; Pan, X.; Li, L.-L.; Tian, J.; Yang, Z.; Lin, X.; Xu, H.-M.; Rudolf, J. D.; Dong, L.-B.*
Chem. Sci., Just accepted
CLICK TO SEE ABSTRACT

64. Canonical terpene synthases in arthropods: intra-phylum gene transfer
Chen, X.; Urban, J. M.; Wurlitzer, J.; Wei. X.; Han, J.; O'Connor, S. E.; Rudolf, J. D.; Köllner, T. G.; Chen, F.
Proc. Natl. Acad. Sci., In press

63. Young investigators in natural products chemistry, biosynthesis, and enzymology
Rudolf, J. D.;*, Barra, L.;*, Awakawa, T.*
Beilstein J. Org. Chem., 2024, 20, 2720–2721
CLICK TO SEE ABSTRACT

62. Exploring and expanding the natural chemical space of bacterial diterpenes
Wei, X.; Ning, W.; McCadden, C. A.; Alsup, T. A.; Li, Z.; Łomowska-Keehner, D. P.; Nafie, J.; Qu, T.; Osei Opoku, M.; Gillia, G. R.; Xu, B.; Icenhour, D. G.; Rudolf, J. D.
ChemRxiv, 2024, doi: https://doi.org/10.26434/chemrxiv-2024-22fhw
CLICK TO SEE ABSTRACT

61. Early-stage biosynthesis of phenalinolactone diterpenoids involves sequential prenylation, epoxidation, and cyclization
Alsup, T. A.; Li, Z.; McCadden, C.; Jagels, A.; Łomowska-Keehner, D. P.; Marshall, E. M.; Dong, L.-B.; Loesgen, S.; Rudolf, J. D.*
RSC Chem. Biol., 2024, 5, 1010–1016
CLICK TO SEE ABSTRACT

60. Chemical diversity of Aspergillus alliaceus phenotypes: discovery of brominated bianthrones with activity against triple-negative breast cancer cell lines
Mandelare, P. E.; Yee, S. S.; Icenhour, D. G.; Kaweesa, E. N.; McCauley, M.; Risinger, A. L.; Rudolf, J. D.; Loesgen, S.*
ChemBioChem, 2024, e202400398
CLICK TO SEE ABSTRACT

59. Computation-guided scaffold exploration of 2E,6E-1,10-trans/cis eunicellanes
Li, Z.; Jindani, S.; Kojasoy, V.; Ortega, T.; Marshall, E.; Abboud, K. A.; Loesgen, S.; Tantillo, D.;* Rudolf, J. D.*
Beilstein J. Org. Chem., 2024, 20, 1320–1326
CLICK TO SEE ABSTRACT

58. Pyrazinone biosynthesis and signaling – myxo style
Rudolf, J. D.;* Loesgen, S.*
ACS Cent. Sci., 2024, 10, 511–513
CLICK TO SEE ABSTRACT

57. Characterization of UbiA terpene synthases with a precursor overproduction system in Escherichia coli
Alsup, T. A.; Osei Opoku, M.; Rudolf, J. D.*
Meth. Enzymol., 2024, 699, 395–417
CLICK TO SEE ABSTRACT

56. Development of platensimycin, platencin, and platensilin overproducers by biosynthetic pathway engineering and fermentation medium optimization
Fluegel, L. L.; Deng, M.-R.; Su, P.; Kalkreuter, E.; Yang, D.; Rudolf, J. D.; Dong, L.-B.; Shen, B.*
J. Ind. Microbiol. Biotechnol., 2024, 51, kuae003
CLICK TO SEE ABSTRACT

55. Class II terpene cyclases: structures, mechanisms, and engineering
Pan, X.; Rudolf, J. D.;* Dong, L.-B.*
Nat. Prod. Rep., 2024, 41, 402–433
CLICK TO SEE ABSTRACT

54. Cryptic isomerization in diterpene biosynthesis and the restoration of an evolutionarily defunct P450
Li, Z.; Xu, B.; Alsup, T. A.; Wei, X.; Ning, W.; Icenhour, D. G.; Ehrenberger, M. A.; Ghiviriga, I.; Giang, B.-D.; Rudolf, J. D.*
J. Am. Chem. Soc., 2023, 145, 22361–22365
CLICK TO SEE ABSTRACT

53. Biosynthesis, enzymology, and future of eunicellane diterpenoids
Li, Z.; Rudolf, J. D.*
J. Ind. Microbiol. Biotechnol., 2023, 50, kuad027
CLICK TO SEE ABSTRACT

52. Cytochrome P450-mediated cyclization in eunicellane-derived diterpenoid biosynthesis
Wang, Z.; Yang, Q.; He, J.; Li, H.; Pan, X.; Li, Z.; Xu, H.-M.; Rudolf, J. D.; Tantillo, D. J.;* Dong, L.-B.*
Angew. Chem., Int. Ed., 2023, 62, e202312490
CLICK TO SEE ABSTRACT

51. First trans-eunicellane terpene synthase in bacteria
Li, Z.; Xu, B.; Kojasoy, V.; Ortega, T.; Adpressa, D. A.; Ning, W.; Wei, X.; Liu, J.; Tantillo, D. J.; Loesgen, S.; Rudolf, J. D.*
Chem, 2023, 9, 698–708
CLICK TO SEE ABSTRACT

50. Discovery, structure, and mechanism of a class II sesquiterpene cyclase
Pan, X.; Du, W.; Zhang, X.; Lin, X.; Li, F.-R.; Yang, Q.; Wang, H.; Rudolf, J. D.;* Zhang, B.* Dong, L.-B.*
J. Am. Chem. Soc., 2022, 144, 22067–22074
CLICK TO SEE ABSTRACT

49. Structure-guided product determination of the bacterial type II diterpene synthase Tpn2
Stowell, E. A.; Ehrenberger, M. E.; Lin, Y.-L.; Chang, C.-Y.;* Rudolf, J. D.*
Commun. Chem., 2022, 5, 146
CLICK TO SEE ABSTRACT

48. Mutation of the eunicellane synthase Bnd4 alters its product profile and expands its prenylation ability
Xu, B.; Ning, W.; Wei, X.; Rudolf, J. D.*
Org. Biomol. Chem., 2022, 20, 8833–8837
CLICK TO SEE ABSTRACT

47. Characterization and structural determination of CmnG-A, the adenylation domain that activates the nonproteinogenic amino acid capreomycidine in capreomycin biosynthesis
Chen, I.-H.; Cheng, T.; Wang, Y.-L.; S.-J. Huang; Hsiao, Y.-H.; Lai, Y.-T.; Rudolf, J. D.; Chang, C.-Y.*
ChemBioChem, 2022, 23, e202200563
CLICK TO SEE ABSTRACT

46. A roadmap for the functional annotation of protein families: a community perspective
de Crecy-Lagard, V.;* [...]; Rudolf, J. D.; [...] (58 total authors)
Database, 2022, baac062
CLICK TO SEE ABSTRACT

45. The structure-function relationship of human bleomycin hydrolase: mutation of a cysteine protease into a serine protease
Zheng, Y.-Z.; Cui, J.; Wang, Y.-L.; Huang, S.-J.; Lin, E.-C.; Huang, S.-C.; Rudolf, J. D.; Yan, X.; Chang, C.-Y.*
ChemBioChem, 2022, 23, e202200186
CLICK TO SEE ABSTRACT

44. The Natural Products Atlas 2.0: a database of microbially-derived natural products
van Santen, J. A.; Poynton, E. F.; Iskakova, D.; McMann, E.; Alsup, T. A.; Clark, T. N.; Fergusson, C. H.; Fewer, D. P.; Hughes, A. H.; McCadden, C. A.; Parra, J.; Soldatou, S.; Rudolf, J. D.; Janssen, E. M-L.; Duncan, K. R.; Linington, R. G.*
Nucl. Acids Res., 2022, 50, D1317–D1323
CLICK TO SEE ABSTRACT

43. Mechanistic insights into the formation of the 6,10-bicyclic eunicellane skeleton by the bacterial diterpene synthase Bnd4
Xu B.; Tantillo, D.; Rudolf, J. D.*
Angew. Chem., Int. Ed., 2021, 60, 23159-23163
CLICK TO SEE ABSTRACT

42. Bacterial diterpene synthases prenylate small molecules
Xu, B.; Li, Z., Alsup, T. A.; Ehrenberger, M. A.; Rudolf, J. D.*
ACS Cat., 2021, 11, 5906–5915
CLICK TO SEE ABSTRACT

41. Discovery and biosynthesis of a structurally dynamic antibacterial diterpenoid
Zhu, C.;‡ Xu, B.;‡ Adpressa, D. A.; Rudolf, J. D.;* Loesgen, S.*
Angew. Chem., Int. Ed., 2021, 60, 14163–14170
CLICK TO SEE ABSTRACT

40. Bacterial terpenome
Rudolf, J. D.;* Alsup, T. A.; Xu, B.; Li, Z.
Nat. Prod. Rep., 2021, 38, 905–980
CLICK TO SEE ABSTRACT

39. Divergent synthesis of complex diterpenes through a hybrid oxidative approach
Zhang, X.; King-Smith, E.; Dong, L.-B.; Yang, L.-C.; Rudolf, J. D.; Shen, B.; Renata, H.*
Science, 2020, 369, 799–806
CLICK TO SEE ABSTRACT

CLICK TO SEE ABSTRACT

37. Characterization and crystal structure of a nonheme diiron monooxygenase involved in platensimycin and platencin biosynthesis
Dong, L.-B.;‡ Liu, Y.-C.;‡ Cepeda, A. J.; Kalkreuter, E.; Deng, M.-R.; Rudolf, J. D.; Chang, C.; Joachimiak, A.; Phillips Jr., G. N.; Shen, B.*
J. Am. Chem. Soc., 2019, 141, 12406-12412
CLICK TO SEE ABSTRACT

36. Evaluation of platensimycin and platensimycin-inspired thioether analogues against methicillin-resistant Staphylococcus aureus in topical and systemic infection mouse models
Su M.; Qiu, L.; Deng, Y.; Ruiz, C. H.; Rudolf, J. D.; Dong, L.-B.; Feng, X.; Cameron, M. D.; Shen, B.; Duan, Y.;* Huang, Y.*
Mol. Pharm., 2019, 16, 3065-3071
CLICK TO SEE ABSTRACT

35. Cryptic and stereospecific hydroxylation, oxidation, and reduction in platensimycin and platencin biosynthesis
Dong, L.-B.;‡ Zhang, X.;‡ Rudolf, J. D.; Deng, M.-R.; Kalkreuter, E.; Cepeda, A. J.; Renata, H.; Shen, B.*
J. Am. Chem. Soc., 2019, 141, 4043–4050
CLICK TO SEE ABSTRACT

34. Cytochrome P450-catalyzed hydroxylation initiating ether formation in platensimycin biosynthesis
Rudolf, J. D.; Dong, L.-B.; Zhang, X.; Renata, H.; Shen, B.*
J. Am. Chem. Soc., 2018, 140, 12349–12353
CLICK TO SEE ABSTRACT

33. Biochemical and structural characterization of TtnD, a prenylated FMN-dependent decarboxylase from the tautomycetin biosynthetic pathway
Annaval, T.; Han, L.; Rudolf, J. D.; Xie, G.; Yang, D.; Chang, C.-Y.; Ma, M.; Crnovcic, I.; Miller, M. D.; Soman, J.; Xu, W.; Phillips Jr., G. N.; Shen, B.*
ACS Chem. Biol., 2018, 13, 2728–2738
CLICK TO SEE ABSTRACT

32. P450-catalyzed tailoring steps in leinamycin biosynthesis featuring regio- and stereoselective hydroxylations and substrate promiscuities
Kwong, T.;‡ Ma, M.;‡ Pan, G.; Hindra; Yang, D.; Lohman, J. R.; Rudolf, J. D.; Cleveland, J. L.; Shen, B.*
Biochemistry, 2018, 57, 5005–5013
CLICK TO SEE ABSTRACT

31. Resistance to enediyne antitumor antibiotics by sequestration
Chang, C.-Y.;‡ Yan, X.;‡ Crnovcic, I.; Annaval, T.; Chang, C.; Nocek, B.; Rudolf, J. D.; Yang, D.; Hindra; Babnigg, G.; Joachimiak, A.; Phillips Jr., G. N.; Shen, B.*
Cell Chem. Biol., 2018, 25, 1075–1085
CLICK TO SEE ABSTRACT

30. Discovery of the tiancilactone antibiotics by genome mining of atypical bacterial type II diterpene synthase
Dong, L.-B..;‡ Rudolf, J. D.;‡ Deng, M.-R.; Yan, X.; Shen, B.*
ChemBioChem, 2018, 19, 1727–1733
CLICK TO SEE ABSTRACT

29. Biosynthesis of thiocarboxylic acid-containing natural products
Dong, L.-B.; Rudolf, J. D.; Kang, D.; Wang, N.; He, C. Q.; Deng, Y.; Huang, Y.; Houk, K. N.; Duan, Y.; Shen, B.*
Nat. Commun., 2018, 9, 2362
CLICK TO SEE ABSTRACT

28. Natural separation of the acyl-CoA ligase reaction results in a non-adenylating enzyme
Wang, N.;‡ Rudolf, J. D.;‡ Dong, L.-B.; Osipiuk, J.; Hatzos-Skintges, C.; Endres, M.; Chang, C.-Y.; Babnigg, G.; Joachimiak, A.; Phillips Jr., G. N.; Shen, B.*
Nat. Chem. Biol., 2018, 14, 730–737
CLICK TO SEE ABSTRACT

27. Structural insights into the free-standing condensation enzyme SgcC5 catalyzing ester-bond formation in the biosynthesis of the enediyne antitumor antibiotic C-1027
Chang, C.-Y.; Lohman, J. R.; Huang, T.; Michalska, K.; Bigelow, L.; Rudolf, J. D.; Jedrzejczak, R.; Yan, Y.; Ma, M.; Babnigg, G.; Joachimiak, A.; Phillips Jr., G. N.; Shen, B.*
Biochemistry, 2018, 57, 3278–3288
CLICK TO SEE ABSTRACT

26. Crystal structure of the thioesterase SgcE10 supporting common polyene intermediates in 9- and 10-membered enediyne core biosynthesis
Annaval, T., Rudolf, J. D.; Chang, C.-Y.; Lohman, J. R.; Kim, Y.; Bigelow, L.; Jedrzejczak, R.; Babnigg, G.; Joachimiak, A.; Phillips Jr., G. N.; Shen, B.*
ACS Omega, 2017, 2, 5159–5169
CLICK TO SEE ABSTRACT

25. Cytochromes P450 for natural product biosynthesis in Streptomyces: sequence, structure, and function
Rudolf, J. D.;‡ Chang, C.-Y.;‡ Ma, M.; Shen, B.*
Nat. Prod. Rep., 2017, 34, 1141–1172
CLICK TO SEE ABSTRACT

24. The role of combinatorial biosynthesis in natural products discovery
Rudolf, J. D.;‡ Crnovcic, I.;‡ Shen, B.;* Newman, D. J.; Cragg, G. M.; Grothaus, P.G., Eds. CRC Press
Chemical Biology of Natural Products, 2017, 87–125

23. Nutritional control of antibiotic production by Streptomyces platensis MA7327: importance of L-aspartic acid
Falzone, M.; Crespo, E.; Jones, K.; Khan, G.; Korn, V.; Patel, A.; Patel, M.; Patel, K.; Perkins, C.; Siddiqui, S.; Stenger, A.; Yu, E.; Gelber, M.; Scheffler, R.; Nayda, V.; Ravin, A.; Komal, R.; Rudolf, J. D.; Shen, B.; Gullo, V.; Demain, A.*
J. Antibiot., 2017, 70, 828–831
CLICK TO SEE ABSTRACT

22. Platensimycin and platencin: inspirations for chemistry, biology, enzymology, and medicine
Rudolf, J. D.; Dong, L.-B.; Shen, B.*
Biochem. Pharm., 2017, 133, 139–151
CLICK TO SEE ABSTRACT

21. Germicidins H–J from Streptomyces sp. CB00361
Ma, M.; Rateb, M. E.; Yang, D.; Rudolf, J. D.; Zhu, X.; Huang, Y.; Zhao, L.-X.; Jiang, Y.; Duan, Y.; Shen, B.*
J. Antibiot., 2017, 70, 200–203

20. In vivo instability of platensimycin and platencin: synthesis and biological evaluation of urea- and carbamate-platensimycin
Dong, L.-D.;‡ Rudolf, J. D.;‡ Lin, L.; Ruiz, C.; Cameron, M. D.; Shen, B.*
Bioorg. Med. Chem., 2017, 25, 1990–1996
CLICK TO SEE ABSTRACT

19. Biosynthetic origin of the ether ring in platensimycin
Rudolf, J. D.;‡ Dong, L.-D.;‡ Manoogian, K.; Shen, B.*
J. Am. Chem. Soc., 2016, 138, 16711–16721
CLICK TO SEE ABSTRACT

18. Strain prioritization and genome mining for enediyne natural products
Yan, X.;‡ Ge, H.-M.;‡ Huang, T.;‡ Hindra;‡ Yang, D.; Teng, Q; Crnovcic, I.; Li, X.; Rudolf, J. D.; Lohman, J. R.; Gansemans, Y.; Zhu, X.; Huang, Y.; Zhao, L.-X.; Jiang, Y.; van Nieuwerburgh, F.; Rader, C.; Duan, Y.;* Shen, B.*
mBio, 2016, 7, e02104–16
TSRI News and ADC Review.">
CLICK TO SEE ABSTRACT

17. A mutasynthetic library of platensimycin and platencin analogues
Dong, L.-B.;‡ Rudolf, J. D.;‡ Shen, B.*
Org. Lett., 2016, 18, 4606–4609
CLICK TO SEE ABSTRACT

16. Crystal structures of SgcE6 and SgcC, the two-component monooxygenase that catalyzes hydroxylation of a carrier protein-tethered substrate during the biosynthesis of the enediyne antitumor antibiotic C-1027 in Streptomyces globisporus
Chang, C.-Y.; Lohman, J. R.; Cao, H.; Tan, K.; Rudolf, J. D.; Ma, M.; Xu, W.; Bingm, C. A.; Yennamalli, R. M.; Bigelow, L.; Babnigg, G.; Yan, X.; Joachimiak, A.; Phillips Jr., G. N.; Shen, B.*
Biochemistry, 2016, 55, 5142–5154
CLICK TO SEE ABSTRACT

15. Structure of the ent-copalyl diphosphate synthase PtmT2 from Streptomyces platensis CB00739, a bacterial type II diterpene synthase
Rudolf, J. D.;‡ Dong, L.-B.;‡ Cao, H.; Hatzos-Skintges, C.; Osipiuk, J.; Endres, M.; Chang, C.-Y.; Ma, M.; Babnigg, G.; Joachimiak, A.; Phillips Jr., G. N.; Shen, B.*
J. Am. Chem. Soc., 2016, 138, 10915–10915
TSRI News, American Laboratory, and Science Newsline.">
CLICK TO SEE ABSTRACT

14. Crystal structure of SgcJ, an NTF2-like superfamily protein involved in biosynthesis of the nine-membered enediyne antitumor antibiotic C-1027
Huang, T.; Chang, C.-Y.; Lohman, J. R.; Rudolf, J. D.; Kim, Y.; Chang, C.; Yang, D.; Ma, M.; Yan, X.; Crnovcic, I.; Bigelow, L.; Clancy, S.; Bingman, C. A.; Yennamalli, R. M.; Babnigg, G.; Joachimiak, A.; Phillips Jr., G. N.; Shen, B.*
J. Antibiot., 2016, 69, 731–740
CLICK TO SEE ABSTRACT

13. Antibacterial sulfur-containing platensimycin and platencin congeners from Streptomyces platensis SB12029
Dong, L.-B.;‡ Rudolf, J. D.;‡ Shen, B.*
Bioorg. Med. Chem., 2016, 24, 6348–6353
CLICK TO SEE ABSTRACT

CLICK TO SEE ABSTRACT

11. Crystal structure of the zorbamycin-binding protein ZbmA, the primary self-resistance element in Streptomyces flavoviridis ATCC21892
Rudolf, J. D.; Bigelow, L.; Chang, C.; Cuff, M. E.; Lohman, J. R.; Chang, C.-Y.; Ma, M.; Yang, D.; Clancy, S.; Babnigg, G.; Joachimiak, A.; Phillips Jr., G. N.; Shen, B.*
Biochemistry, 2015, 54, 6842–6851
CLICK TO SEE ABSTRACT

10. Angucyclines and angucyclinones from Streptomyces sp. CB01913 featuring C-ring cleavage and expansion
Ma, M.; Rateb, M., Teng, Q., Yang, D., Rudolf, J. D.; Zhu, X.; Huang, Y.; Zhao, L.-X.; Jiang, Y.; Li, X.; Rader, C.; Duan, Y.; Shen, B.*
J. Nat. Prod., 2015, 78, 2471–2480
CLICK TO SEE ABSTRACT

2015 Hot Article in Mol. BioSyst.; 10th Anniversary of Mol. BioSyst. cover article.Cover">
CLICK TO SEE ABSTRACT

8. Enediynes: exploration of microbial genomics to discover new anticancer drug leads
Shen, B.;* Hindra; Yan, X.; Huang, T.; Ge, H.-M.; Yang, D.; Qihui, T.; Rudolf, J. D.; Lohman, J. R.
Bioorg. Med. Chem. Lett., 2015, 25, 9-15
CLICK TO SEE ABSTRACT

7. BlmB and TlmB provide resistance to the bleomycin family of antitumor antibiotics by N-acetylating metal-free bleomycin, tallysomycin, phleomycin, and zorbamycin
Coughlin, J. M.;‡ Rudolf, J. D.;‡ Wang, L.; Galm, U.; Wendt-Pienkowsky, E.; Tao, M.; Shen, B.*
Biochemistry, 2014, 53, 6901–6909
CLICK TO SEE ABSTRACT

6. Strain prioritization for natural product discovery by a high-throughput real-time PCR method
Hindra;‡ Huang, T.;‡ Yang, D.;‡ Rudolf, J. D.;‡ Xie, P.; Xie, G.; Teng, Q.; Lohman, J. R.; Zhu, X.; Huang, Y.; Zhao, L.-X.; Jiang, Y.; Duan, Y.;* Shen, B.*
Nat. Prod., 2014, 77, 2296–2303
C&E News, Oct. 6, 2014.">
CLICK TO SEE ABSTRACT

5. Enediyne polyketide synthases stereospecifically reduce the β-ketoacyl intermediates to β-D-hydroxyacyl intermediates in enediyne core biosynthesis
Ge, H.-M.; Huang, T.; Rudolf, J. D.; Lohman, J. R.; Huang, S.-X.; Guo, X.; Shen, B.*
Org. Lett., 2014, 16, 3958–3961
CLICK TO SEE ABSTRACT

4. Biosynthetic potential-based strain prioritization for natural product discovery – a showcase for diterpenoid producing actinomycetes
Xie, P.;‡ Ma, M.;‡ Rateb, M.; Shaaban, K.; Yu, Z.; Huang, S.-X.; Zhao, L.-X.; Zhu, X.; Yan, Y.; Peterson, R. M.; Lohman, J. R.; Yan, D.; Yin, M.; Rudolf, J. D.; Jiang, Y.; Duan, Y.; Shen, B.*
J. Nat. Prod., 2014, 77, 377–387
CLICK TO SEE ABSTRACT

3. Mechanisms of self-resistance in the platensimycin and platencin producing Streptomyces platensis MA7327 and MA7339 strains
Peterson, R. M.;‡ Huang, T.;‡ Rudolf, J. D.; Smanski, M. J.; Shen, B.*
Chem. Biol., 2014, 21, 389–397
TSRI News, The Scientist Magazine, and Genetic Eng. & Biotech. News.">
CLICK TO SEE ABSTRACT

CLICK TO SEE ABSTRACT

1. Multisite prenylation of 4-substituted tryptophans by dimethylallyltryptophan synthase
Rudolf, J. D.; Wang, H.; Poulter, C. D.*
J. Am. Chem. Soc., 2013, 135, 1895–1902
CLICK TO SEE ABSTRACT

23. Biosynthesis of bacterial meroterpenoid reveals a non-canonical class II terpene cyclase
Wang, Z.; Alsup, T. A.; Pan, X.; Li, L.-L.; Tian, J.; Yang, Z.; Lin, X.; Xu, H.-M.; Rudolf, J. D.; Dong, L.-B.*
Chem. Sci., Just accepted
CLICK TO SEE ABSTRACT

22. Canonical terpene synthases in arthropods: intra-phylum gene transfer
Chen, X.; Urban, J. M.; Wurlitzer, J.; Wei. X.; Han, J.; O'Connor, S. E.; Rudolf, J. D.; Köllner, T. G.; Chen, F.
Proc. Natl. Acad. Sci., In press

21. Exploring and expanding the natural chemical space of bacterial diterpenes
Wei, X.; Ning, W.; McCadden, C. A.; Alsup, T. A.; Li, Z.; Łomowska-Keehner, D. P.; Nafie, J.; Qu, T.; Osei Opoku, M.; Gillia, G. R.; Xu, B.; Icenhour, D. G.; Rudolf, J. D.
ChemRxiv, 2024, doi: https://doi.org/10.26434/chemrxiv-2024-22fhw
CLICK TO SEE ABSTRACT

20. Early-stage biosynthesis of phenalinolactone diterpenoids involves sequential prenylation, epoxidation, and cyclization
Alsup, T. A.; Li, Z.; McCadden, C.; Jagels, A.; Łomowska-Keehner, D. P.; Marshall, E. M.; Dong, L.-B.; Loesgen, S.; Rudolf, J. D.*
RSC Chem. Biol., 2024, 5, 1010–1016
CLICK TO SEE ABSTRACT

19. Chemical diversity of Aspergillus alliaceus phenotypes: discovery of brominated bianthrones with activity against triple-negative breast cancer cell lines
Mandelare, P. E.; Yee, S. S.; Icenhour, D. G.; Kaweesa, E. N.; McCauley, M.; Risinger, A. L.; Rudolf, J. D.; Loesgen, S.*
ChemBioChem, 2024, e202400398
CLICK TO SEE ABSTRACT

18. Computation-guided scaffold exploration of 2E,6E-1,10-trans/cis eunicellanes
Li, Z.; Jindani, S.; Kojasoy, V.; Ortega, T.; Marshall, E.; Abboud, K. A.; Loesgen, S.; Tantillo, D.;* Rudolf, J. D.*
Beilstein J. Org. Chem., 2024, 20, 1320–1326
CLICK TO SEE ABSTRACT

17. Class II terpene cyclases: structures, mechanisms, and engineering
Pan, X.; Rudolf, J. D.;* Dong, L.-B.*
Nat. Prod. Rep., 2024, 41, 402–433
CLICK TO SEE ABSTRACT

16. Cryptic isomerization in diterpene biosynthesis and the restoration of an evolutionarily defunct P450
Li, Z.; Xu, B.; Alsup, T. A.; Wei, X.; Ning, W.; Icenhour, D. G.; Ehrenberger, M. A.; Ghiviriga, I.; Giang, B.-D.; Rudolf, J. D.*
J. Am. Chem. Soc., 2023, 145, 22361–22365
CLICK TO SEE ABSTRACT

15. Biosynthesis, enzymology, and future of eunicellane diterpenoids
Li, Z.; Rudolf, J. D.*
J. Ind. Microbiol. Biotechnol., 2023, 50, kuad027
CLICK TO SEE ABSTRACT

14. Cytochrome P450-mediated cyclization in eunicellane-derived diterpenoid biosynthesis
Wang, Z.; Yang, Q.; He, J.; Li, H.; Pan, X.; Li, Z.; Xu, H.-M.; Rudolf, J. D.; Tantillo, D. J.;* Dong, L.-B.*
Angew. Chem., Int. Ed., 2023, 62, e202312490
CLICK TO SEE ABSTRACT

13. First trans-eunicellane terpene synthase in bacteria
Li, Z.; Xu, B.; Kojasoy, V.; Ortega, T.; Adpressa, D. A.; Ning, W.; Wei, X.; Liu, J.; Tantillo, D. J.; Loesgen, S.; Rudolf, J. D.*
Chem, 2023, 9, 698–708
CLICK TO SEE ABSTRACT

12. Discovery, structure, and mechanism of a class II sesquiterpene cyclase
Pan, X.; Du, W.; Zhang, X.; Lin, X.; Li, F.-R.; Yang, Q.; Wang, H.; Rudolf, J. D.;* Zhang, B.* Dong, L.-B.*
J. Am. Chem. Soc., 2022, 144, 22067–22074
CLICK TO SEE ABSTRACT

11. Structure-guided product determination of the bacterial type II diterpene synthase Tpn2
Stowell, E. A.; Ehrenberger, M. E.; Lin, Y.-L.; Chang, C.-Y.;* Rudolf, J. D.*
Commun. Chem., 2022, 5, 146
CLICK TO SEE ABSTRACT

10. Mutation of the eunicellane synthase Bnd4 alters its product profile and expands its prenylation ability
Xu, B.; Ning, W.; Wei, X.; Rudolf, J. D.*
Org. Biomol. Chem., 2022, 20, 8833–8837
CLICK TO SEE ABSTRACT

9. Characterization and structural determination of CmnG-A, the adenylation domain that activates the nonproteinogenic amino acid capreomycidine in capreomycin biosynthesis
Chen, I.-H.; Cheng, T.; Wang, Y.-L.; S.-J. Huang; Hsiao, Y.-H.; Lai, Y.-T.; Rudolf, J. D.; Chang, C.-Y.*
ChemBioChem, 2022, 23, e202200563
CLICK TO SEE ABSTRACT

8. A roadmap for the functional annotation of protein families: a community perspective
de Crecy-Lagard, V.;* [...]; Rudolf, J. D.; [...] (58 total authors)
Database, 2022, baac062
CLICK TO SEE ABSTRACT

7. The structure-function relationship of human bleomycin hydrolase: mutation of a cysteine protease into a serine protease
Zheng, Y.-Z.; Cui, J.; Wang, Y.-L.; Huang, S.-J.; Lin, E.-C.; Huang, S.-C.; Rudolf, J. D.; Yan, X.; Chang, C.-Y.*
ChemBioChem, 2022, 23, e202200186
CLICK TO SEE ABSTRACT

6. The Natural Products Atlas 2.0: a database of microbially-derived natural products
van Santen, J. A.; Poynton, E. F.; Iskakova, D.; McMann, E.; Alsup, T. A.; Clark, T. N.; Fergusson, C. H.; Fewer, D. P.; Hughes, A. H.; McCadden, C. A.; Parra, J.; Soldatou, S.; Rudolf, J. D.; Janssen, E. M-L.; Duncan, K. R.; Linington, R. G.*
Nucl. Acids Res., 2022, 50, D1317–D1323
CLICK TO SEE ABSTRACT

5. Mechanistic insights into the formation of the 6,10-bicyclic eunicellane skeleton by the bacterial diterpene synthase Bnd4
Xu B.; Tantillo, D.; Rudolf, J. D.*
Angew. Chem., Int. Ed., 2021, 60, 23159-23163
CLICK TO SEE ABSTRACT

4. Bacterial diterpene synthases prenylate small molecules
Xu, B.; Li, Z., Alsup, T. A.; Ehrenberger, M. A.; Rudolf, J. D.*
ACS Cat., 2021, 11, 5906–5915
CLICK TO SEE ABSTRACT

3. Discovery and biosynthesis of a structurally dynamic antibacterial diterpenoid
Zhu, C.;‡ Xu, B.;‡ Adpressa, D. A.; Rudolf, J. D.;* Loesgen, S.*
Angew. Chem., Int. Ed., 2021, 60, 14163–14170
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2. Bacterial terpenome
Rudolf, J. D.;* Alsup, T. A.; Xu, B.; Li, Z.
Nat. Prod. Rep., 2021, 38, 905–980
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38. Development of platensimycin, platencin, and platensilin overproducers by biosynthetic pathway engineering and fermentation medium optimization
Fluegel, L. L.; Deng, M.-R.; Su, P.; Kalkreuter, E.; Yang, D.; Rudolf, J. D.; Dong, L.-B.; Shen, B.*
J. Ind. Microbiol. Biotechnol., 2024, 51, kuae003
CLICK TO SEE ABSTRACT

37. Divergent synthesis of complex diterpenes through a hybrid oxidative approach
Zhang, X.; King-Smith, E.; Dong, L.-B.; Yang, L.-C.; Rudolf, J. D.; Shen, B.; Renata, H.*
Science, 2020, 369, 799–806
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36. Characterization and crystal structure of a nonheme diiron monooxygenase involved in platensimycin and platencin biosynthesis
Dong, L.-B.;‡ Liu, Y.-C.;‡ Cepeda, A. J.; Kalkreuter, E.; Deng, M.-R.; Rudolf, J. D.; Chang, C.; Joachimiak, A.; Phillips Jr., G. N.; Shen, B.*
J. Am. Chem. Soc., 2019, 141, 12406-12412
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35. Evaluation of platensimycin and platensimycin-inspired thioether analogues against methicillin-resistant Staphylococcus aureus in topical and systemic infection mouse models
Su M.; Qiu, L.; Deng, Y.; Ruiz, C. H.; Rudolf, J. D.; Dong, L.-B.; Feng, X.; Cameron, M. D.; Shen, B.; Duan, Y.;* Huang, Y.*
Mol. Pharm., 2019, 16, 3065-3071
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34. Cryptic and stereospecific hydroxylation, oxidation, and reduction in platensimycin and platencin biosynthesis
Dong, L.-B.;‡ Zhang, X.;‡ Rudolf, J. D.; Deng, M.-R.; Kalkreuter, E.; Cepeda, A. J.; Renata, H.; Shen, B.*
J. Am. Chem. Soc., 2019, 141, 4043–4050
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33. Cytochrome P450-catalyzed hydroxylation initiating ether formation in platensimycin biosynthesis
Rudolf, J. D.; Dong, L.-B.; Zhang, X.; Renata, H.; Shen, B.*
J. Am. Chem. Soc., 2018, 140, 12349–12353
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32. Biochemical and structural characterization of TtnD, a prenylated FMN-dependent decarboxylase from the tautomycetin biosynthetic pathway
Annaval, T.; Han, L.; Rudolf, J. D.; Xie, G.; Yang, D.; Chang, C.-Y.; Ma, M.; Crnovcic, I.; Miller, M. D.; Soman, J.; Xu, W.; Phillips Jr., G. N.; Shen, B.*
ACS Chem. Biol., 2018, 13, 2728–2738
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31. P450-catalyzed tailoring steps in leinamycin biosynthesis featuring regio- and stereoselective hydroxylations and substrate promiscuities
Kwong, T.;‡ Ma, M.;‡ Pan, G.; Hindra; Yang, D.; Lohman, J. R.; Rudolf, J. D.; Cleveland, J. L.; Shen, B.*
Biochemistry, 2018, 57, 5005–5013
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30. Resistance to enediyne antitumor antibiotics by sequestration
Chang, C.-Y.;‡ Yan, X.;‡ Crnovcic, I.; Annaval, T.; Chang, C.; Nocek, B.; Rudolf, J. D.; Yang, D.; Hindra; Babnigg, G.; Joachimiak, A.; Phillips Jr., G. N.; Shen, B.*
Cell Chem. Biol., 2018, 25, 1075–1085
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29. Discovery of the tiancilactone antibiotics by genome mining of atypical bacterial type II diterpene synthase
Dong, L.-B..;‡ Rudolf, J. D.;‡ Deng, M.-R.; Yan, X.; Shen, B.*
ChemBioChem, 2018, 19, 1727–1733
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28. Biosynthesis of thiocarboxylic acid-containing natural products
Dong, L.-B.; Rudolf, J. D.; Kang, D.; Wang, N.; He, C. Q.; Deng, Y.; Huang, Y.; Houk, K. N.; Duan, Y.; Shen, B.*
Nat. Commun., 2018, 9, 2362
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27. Natural separation of the acyl-CoA ligase reaction results in a non-adenylating enzyme
Wang, N.;‡ Rudolf, J. D.;‡ Dong, L.-B.; Osipiuk, J.; Hatzos-Skintges, C.; Endres, M.; Chang, C.-Y.; Babnigg, G.; Joachimiak, A.; Phillips Jr., G. N.; Shen, B.*
Nat. Chem. Biol., 2018, 14, 730–737
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26. Structural insights into the free-standing condensation enzyme SgcC5 catalyzing ester-bond formation in the biosynthesis of the enediyne antitumor antibiotic C-1027
Chang, C.-Y.; Lohman, J. R.; Huang, T.; Michalska, K.; Bigelow, L.; Rudolf, J. D.; Jedrzejczak, R.; Yan, Y.; Ma, M.; Babnigg, G.; Joachimiak, A.; Phillips Jr., G. N.; Shen, B.*
Biochemistry, 2018, 57, 3278–3288
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25. Crystal structure of the thioesterase SgcE10 supporting common polyene intermediates in 9- and 10-membered enediyne core biosynthesis
Annaval, T., Rudolf, J. D.; Chang, C.-Y.; Lohman, J. R.; Kim, Y.; Bigelow, L.; Jedrzejczak, R.; Babnigg, G.; Joachimiak, A.; Phillips Jr., G. N.; Shen, B.*
ACS Omega, 2017, 2, 5159–5169
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24. Cytochromes P450 for natural product biosynthesis in Streptomyces: sequence, structure, and function
Rudolf, J. D.;‡ Chang, C.-Y.;‡ Ma, M.; Shen, B.*
Nat. Prod. Rep., 2017, 34, 1141–1172
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23. Nutritional control of antibiotic production by Streptomyces platensis MA7327: importance of L-aspartic acid
Falzone, M.; Crespo, E.; Jones, K.; Khan, G.; Korn, V.; Patel, A.; Patel, M.; Patel, K.; Perkins, C.; Siddiqui, S.; Stenger, A.; Yu, E.; Gelber, M.; Scheffler, R.; Nayda, V.; Ravin, A.; Komal, R.; Rudolf, J. D.; Shen, B.; Gullo, V.; Demain, A.*
J. Antibiot., 2017, 70, 828–831
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22. Platensimycin and platencin: inspirations for chemistry, biology, enzymology, and medicine
Rudolf, J. D.; Dong, L.-B.; Shen, B.*
Biochem. Pharm., 2017, 133, 139–151
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21. Germicidins H–J from Streptomyces sp. CB00361
Ma, M.; Rateb, M. E.; Yang, D.; Rudolf, J. D.; Zhu, X.; Huang, Y.; Zhao, L.-X.; Jiang, Y.; Duan, Y.; Shen, B.*
J. Antibiot., 2017, 70, 200–203

20. In vivo instability of platensimycin and platencin: synthesis and biological evaluation of urea- and carbamate-platensimycin
Dong, L.-D.;‡ Rudolf, J. D.;‡ Lin, L.; Ruiz, C.; Cameron, M. D.; Shen, B.*
Bioorg. Med. Chem., 2017, 25, 1990–1996
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19. Biosynthetic origin of the ether ring in platensimycin
Rudolf, J. D.;‡ Dong, L.-D.;‡ Manoogian, K.; Shen, B.*
J. Am. Chem. Soc., 2016, 138, 16711–16721
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18. Strain prioritization and genome mining for enediyne natural products
Yan, X.;‡ Ge, H.-M.;‡ Huang, T.;‡ Hindra;‡ Yang, D.; Teng, Q; Crnovcic, I.; Li, X.; Rudolf, J. D.; Lohman, J. R.; Gansemans, Y.; Zhu, X.; Huang, Y.; Zhao, L.-X.; Jiang, Y.; van Nieuwerburgh, F.; Rader, C.; Duan, Y.;* Shen, B.*
mBio, 2016, 7, e02104–16
TSRI News and ADC Review.">
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17. A mutasynthetic library of platensimycin and platencin analogues
Dong, L.-B.;‡ Rudolf, J. D.;‡ Shen, B.*
Org. Lett., 2016, 18, 4606–4609
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16. Crystal structures of SgcE6 and SgcC, the two-component monooxygenase that catalyzes hydroxylation of a carrier protein-tethered substrate during the biosynthesis of the enediyne antitumor antibiotic C-1027 in Streptomyces globisporus
Chang, C.-Y.; Lohman, J. R.; Cao, H.; Tan, K.; Rudolf, J. D.; Ma, M.; Xu, W.; Bingm, C. A.; Yennamalli, R. M.; Bigelow, L.; Babnigg, G.; Yan, X.; Joachimiak, A.; Phillips Jr., G. N.; Shen, B.*
Biochemistry, 2016, 55, 5142–5154
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15. Structure of the ent-copalyl diphosphate synthase PtmT2 from Streptomyces platensis CB00739, a bacterial type II diterpene synthase
Rudolf, J. D.;‡ Dong, L.-B.;‡ Cao, H.; Hatzos-Skintges, C.; Osipiuk, J.; Endres, M.; Chang, C.-Y.; Ma, M.; Babnigg, G.; Joachimiak, A.; Phillips Jr., G. N.; Shen, B.*
J. Am. Chem. Soc., 2016, 138, 10915–10915
TSRI News, American Laboratory, and Science Newsline.">
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14. Crystal structure of SgcJ, an NTF2-like superfamily protein involved in biosynthesis of the nine-membered enediyne antitumor antibiotic C-1027
Huang, T.; Chang, C.-Y.; Lohman, J. R.; Rudolf, J. D.; Kim, Y.; Chang, C.; Yang, D.; Ma, M.; Yan, X.; Crnovcic, I.; Bigelow, L.; Clancy, S.; Bingman, C. A.; Yennamalli, R. M.; Babnigg, G.; Joachimiak, A.; Phillips Jr., G. N.; Shen, B.*
J. Antibiot., 2016, 69, 731–740
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13. Antibacterial sulfur-containing platensimycin and platencin congeners from Streptomyces platensis SB12029
Dong, L.-B.;‡ Rudolf, J. D.;‡ Shen, B.*
Bioorg. Med. Chem., 2016, 24, 6348–6353
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11. Crystal structure of the zorbamycin-binding protein ZbmA, the primary self-resistance element in Streptomyces flavoviridis ATCC21892
Rudolf, J. D.; Bigelow, L.; Chang, C.; Cuff, M. E.; Lohman, J. R.; Chang, C.-Y.; Ma, M.; Yang, D.; Clancy, S.; Babnigg, G.; Joachimiak, A.; Phillips Jr., G. N.; Shen, B.*
Biochemistry, 2015, 54, 6842–6851
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10. Angucyclines and angucyclinones from Streptomyces sp. CB01913 featuring C-ring cleavage and expansion
Ma, M.; Rateb, M., Teng, Q., Yang, D., Rudolf, J. D.; Zhu, X.; Huang, Y.; Zhao, L.-X.; Jiang, Y.; Li, X.; Rader, C.; Duan, Y.; Shen, B.*
J. Nat. Prod., 2015, 78, 2471–2480
CLICK TO SEE ABSTRACT

2015 Hot Article in Mol. BioSyst.; 10th Anniversary of Mol. BioSyst. cover article.Cover">
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8. Enediynes: exploration of microbial genomics to discover new anticancer drug leads
Shen, B.;* Hindra; Yan, X.; Huang, T.; Ge, H.-M.; Yang, D.; Qihui, T.; Rudolf, J. D.; Lohman, J. R.
Bioorg. Med. Chem. Lett., 2015, 25, 9-15
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7. BlmB and TlmB provide resistance to the bleomycin family of antitumor antibiotics by N-acetylating metal-free bleomycin, tallysomycin, phleomycin, and zorbamycin
Coughlin, J. M.;‡ Rudolf, J. D.;‡ Wang, L.; Galm, U.; Wendt-Pienkowsky, E.; Tao, M.; Shen, B.*
Biochemistry, 2014, 53, 6901–6909
CLICK TO SEE ABSTRACT

6. Strain prioritization for natural product discovery by a high-throughput real-time PCR method
Hindra;‡ Huang, T.;‡ Yang, D.;‡ Rudolf, J. D.;‡ Xie, P.; Xie, G.; Teng, Q.; Lohman, J. R.; Zhu, X.; Huang, Y.; Zhao, L.-X.; Jiang, Y.; Duan, Y.;* Shen, B.*
Nat. Prod., 2014, 77, 2296–2303
C&E News, Oct. 6, 2014.">
CLICK TO SEE ABSTRACT

5. Enediyne polyketide synthases stereospecifically reduce the β-ketoacyl intermediates to β-D-hydroxyacyl intermediates in enediyne core biosynthesis
Ge, H.-M.; Huang, T.; Rudolf, J. D.; Lohman, J. R.; Huang, S.-X.; Guo, X.; Shen, B.*
Org. Lett., 2014, 16, 3958–3961
CLICK TO SEE ABSTRACT

4. Biosynthetic potential-based strain prioritization for natural product discovery – a showcase for diterpenoid producing actinomycetes
Xie, P.;‡ Ma, M.;‡ Rateb, M.; Shaaban, K.; Yu, Z.; Huang, S.-X.; Zhao, L.-X.; Zhu, X.; Yan, Y.; Peterson, R. M.; Lohman, J. R.; Yan, D.; Yin, M.; Rudolf, J. D.; Jiang, Y.; Duan, Y.; Shen, B.*
J. Nat. Prod., 2014, 77, 377–387
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3. Mechanisms of self-resistance in the platensimycin and platencin producing Streptomyces platensis MA7327 and MA7339 strains
Peterson, R. M.;‡ Huang, T.;‡ Rudolf, J. D.; Smanski, M. J.; Shen, B.*
Chem. Biol., 2014, 21, 389–397
TSRI News, The Scientist Magazine, and Genetic Eng. & Biotech. News.">
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1. Multisite prenylation of 4-substituted tryptophans by dimethylallyltryptophan synthase
Rudolf, J. D.; Wang, H.; Poulter, C. D.*
J. Am. Chem. Soc., 2013, 135, 1895–1902
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2. Characterization of UbiA terpene synthases with a precursor overproduction system in Escherichia coli
Alsup, T. A.; Osei Opoku, M.; Rudolf, J. D.*
Meth. Enzymol., 2024, 699, 395–417
CLICK TO SEE ABSTRACT

1. The role of combinatorial biosynthesis in natural products discovery
Rudolf, J. D.;‡ Crnovcic, I.;‡ Shen, B.;* Newman, D. J.; Cragg, G. M.; Grothaus, P.G., Eds. CRC Press
Chemical Biology of Natural Products, 2017, 87–125

2. Young investigators in natural products chemistry, biosynthesis, and enzymology
Rudolf, J. D.;*, Barra, L.;*, Awakawa, T.*
Beilstein J. Org. Chem., 2024, 20, 2720–2721
CLICK TO SEE ABSTRACT

1. Pyrazinone biosynthesis and signaling – myxo style
Rudolf, J. D.;* Loesgen, S.*
ACS Cent. Sci., 2024, 10, 511–513
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